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KMID : 0358419960390020328
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Korean Journal of Obstetrics and Gynecology
1996 Volume.39 No. 2 p.328 ~ p.345
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The Effects of Growth Factors on the Production of Insulin-like Growth Factors and Their Binding Proteins by Human Luteinizing Granulosa Cells
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김정구
김석현/최영민/신창재/문신용/장윤석/이진용
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Abstract
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Intraovarian polypeptide growth factors such as insulin-like growth factors(IGFs), epidermal growth factor(EGF), transforming growth factor beta(TGF β) and basic fibroblastic growth factor(bFGF) have been implicated as potentiators or mediators
of
gonadotropin action in the ovarian cycle. This study was undertaken to evaluate the effect of EGF, TGF βand bFGF on the production of IGFs and IGF-binding proteins(IGFBPs) in human luteinizing granulosa cells cultured in serum free medium. IGFs
were
measured by double antibody radioimmunoassay and IGFBPs by Western ligand blotting. No immunoreactive IGF-I was detectable in all conditioned media of luteinizing granulosa cells. IGF-I(1~100ng/ml) and bFGF(100 ng/ml) were found to stimulate
IGFBP-3 and
IGFBP-2 production significantly. Also bFGF had similar effect on the IGF-II production but IGF-I had not show this effect. On the other hand IGFBP-2, IGFBP-3 and IGF-II production were significantly inhibited by TGFβ(1~10ng/ml). EGF was shown
to
have
biphasic effect on the IGFBPs production and stimulated IGF-II production at a concentration of 10~100ng/ml. Treatment of luteinizing granulosa cells cultures with IGF-I(10ng/ml) and TGFβ(0.1~1ng/ml) significantly enhanced IGFBP-3 levels in
conditioned
media but exerted no significant effect on IGFBP-2 and IGF-II levels. EGF(10 ng/ml) plus TGFβ(1 ng/ml) significantly inhibited these IGFBPs production but resulted in significant increase in IGF-II production. Coincubation of luteinizing
granulosa
cells with IGF, EGF and/ or TGFβsignificantly(p<0.005) stimulated IGFBP-2, IGFBP-3 and IGF-II production. Our data indicate that IGF-I-induced increases in IGFBPs may play an important role in controlling local cell responsiveness to this growth
factor
and that axis between IGF-II, IGFBP and other growth factors may present.
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KEYWORD
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